Comparison between two different methods of immobilizing NGF in poly(DL-lactic acid-co-glycolic acid) conduit for peripheral nerve regeneration by EDC/NHS/MES and genipin

勤益科大機構典藏 > 工程學院 > 化工與材料工程系(所) > 【化工與材料工程系】期刊論文 > Item 987654321/6636

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncut.edu.tw/handle/987654321/6636

题名:	Comparison between two different methods of immobilizing NGF in poly(DL-lactic acid-co-glycolic acid) conduit for peripheral nerve regeneration by EDC/NHS/MES and genipin
作者:	謝淑枝
贡献者:	化工與材料工程系
日期:	2011-12
上传时间:	2017-11-14 13:51:52 (UTC+8)
摘要:	For surface modification and nerve regeneration, chitosan, followed by nerve growth factor (NGF), was immobilized onto the interior surface of poly (lactic acit-co-glycolic) conduits, using EDC/NHS/MES system (EDCs) and genipin (GP). Four new conduits were, therefore, obtained and named by immobilizing order-EDCs/EDCs, GP/EDCs, EDCs/GP, and GP/GP groups. The immobilized methods used were evaluated and compared, respectively. The researchers found that the EDCs- and GP-cross-linked chitosan displayed higher hydrophilic than pure poly (DL-lactic acid-co-glycolic acid) (PLGA) in water contact angle experiment, which meant the cell compatibility was improved by the modification. Scanning electron microscopic observations revealed that the GP-cross-linking of chitosan greatly improved cell compatibility while cultured rat PC12 cells were flatter and more spindle-shaped than EDCs-cross-linked chitosan. The results concerning the GP-cross-linked chitosan revealed significant proliferation of the seeded cells relative to pure PLGA films, as determined by counting cells and MTT assay. The NGF was released from the modified conduits in two separate periods—an initial burst in 5 days and then slow release from day 10 to day 40. The GP/EDCs group had the highest NGF value among all groups after the 5th day. Finally, the controlled-release conduits were used to bridge a 10 mm rat sciatic nerve defect. Six weeks following implantation, morphological analysis revealed the highest numbers of myelinated axons in the midconduit and distal regenerated nerve in GP/EDCs group. Therefore, the results confirm that GP/EDCs groups with good cell compatibility and effective release of NGF can considerably improve peripheral nerve regeneration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
關聯:	Journal of Biomedical Materials Research Part A
显示于类别:	[化工與材料工程系(所)] 【化工與材料工程系】期刊論文

文件中的档案:

没有与此文件相关的档案.

检视Licence

在NCUTIR中所有的数据项都受到原著作权保护.

数据加载中.....