The objective of this paper is to make the investigation on energy efficiency of the air conditioning via the viewpoint of the vents arrangement. In the research, a real typical convenience store located in Taichung, Taiwan is selected as a case study. The computational fluid dynamic (CFD) technique is adopted to simulate the flow, thermal, and humidity distribution inside the convenient store under both close and open automatic door conditions. The results shows that good air conditioning vents arrangement could lead the flow going through the path which could make sufficient heat exchange with the indoor air and increase the energy efficiency. The short-path should be avoided to escape the air goes though one inlet vent to the one outlet vent directly such that the fresh air could not make mixture and heat exchange with the indoor air. The present methodology of integrating CFD simulation and experiments is powerful to make fast and accurate design on the design of air conditioning vents layout. (14 refs)