The production of bacterial cellulose (BC) from Gluconacetobacter xylinus could be improved by the Taguchi method. Both the initial pH and glucose concentration are the important factors to affect the production of the BC. The optimum combination of these factors and levels is the G. xylinus ATCC 23768, YPD as the basic growth medium, initial pH=4.5, glucose concentration = 5% (w/v), acetic acid concentration= 1.5% (v/v) and liquid height=7.2 cm. After the modified of factors and the levels, the maximum BC concentration and wet film thickness could be increased 37.5% to 0.557 g-dry cellulose/L and 39.0% to 3.92 mm, respectively.
